分类目录归档:有关搬砖的事情

3/27 分子生物学实验思考题

有一种想在这里更新思考题答案的冲动,要是有人帮我把答案附在评论里就更好了~

1 PCR扩增仪的热盖有什么作用?

You know when you use a heatblock with no lid and all your sample ends condensing in the lid of the tube? That's why they invented the heated lid. It will discourage your sample to evaporate and condensate on the lid, where the temperature is lower than what you wanted it to be. In the old days of PCR, when heated lids were not available yet, every 95oC cycle would cause evaporation and loss of sample, so after 25 cycles, you had nothing on the bottom of the tube. So they overlaid a droplet of mineral oil on the reaction, so that it wouldn't evaporate, then they had to extract the reaction without the oil, that was probably a big pain! Then a very smart person invented the heated lid, fixing everything.Use the heated lid, it won't hurt your reaction. It doesn't even need to be a high temperature, just high enough to discourage condensing.

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